Microbial Diversity and Pharmaceutical Biotransformations

Collaborators: Lauren B. Stadler

Stadler, L.B.†, Delgado Vela, J. †, Jain, S., Dick, G., and Love, N. G. Elucidating the Relationship between Wastewater Treatment Plant Microbial Diversity and Pharmaceutical Fate. 2017. Microbial Biotechnology. DOI: 10.1111/1751-7915.12870

Research Questions

  • Do functional and taxonomic diversity associate with each other in wastewater treatment plant microbial communities?
  • How does wastewater treatment plant microbial diversity impact pharmaceutical biotransformations?
  • Which functions associate with pharmaceutical biotransformations?

Research Approach

Established diversity gradient using wastewater microbial community

Regrew diluted cultures and performed pharamceutical biotransformation assays. 

Analyzed sequencing data, amplicon sequencing of the 16S rRNA gene and 16S rRNA, as well as whole community metagenomics and metatranscriptomics,  to identify the functions and taxonomic groups that were lost in the dilution gradient. 

  Counts of significantly differentially expressed genes (likelihood ratio,  p adj < 0.05) that were also significantly associated with EE2 biotransformation (Spearman,  p  < 0.05). The vertical bar on the left shows monooxygenase genes (grey) and hydroxylase genes (blue). Genes with the same name are unique sequences that were annotated with the same KEGG Orthology. 

Counts of significantly differentially expressed genes (likelihood ratio, padj < 0.05) that were also significantly associated with EE2 biotransformation (Spearman, p < 0.05). The vertical bar on the left shows monooxygenase genes (grey) and hydroxylase genes (blue). Genes with the same name are unique sequences that were annotated with the same KEGG Orthology.