Sulfide in a membrane aerated biofilm reactor 

Delgado Vela, J., Gordon, K. J., Bekele, Z.A., Klatt, J.M., Dick, G.J., Love, N.G.  The impact of sulfide on the performance of a membrane aerated biofilm reactor treating mainstream anaerobic effluent. In preparation for submission to Environmental Science and Technology.

Delgado Vela, J., Bristow L.A., Marchant, H.K., Love, N.G., Dick, G.J. Sulfide changes microbial interactions in a nitrogen cycling biofilm reactor. In preparation for submission to Environmental Microbiology

Research questions

  • How does a MABR respond to long and short-term sulfide doses?
  • How do nitrifying populations respond to sulfide?
  • How does sulfide impact nitrous oxide emissions from the reactor? 

Research Approach


Long term impact of sulfide 

Measured the changes in nitrification due to increases in sulfide over the long term 


Short term impact of sulfide

Measured changes in nitrification due to short term (24 hour) pulses of sulfide

Also assessed the impacts of sulfide using microsensor profiles (dissolved oxygen, hydrogen sulfide, pH, and nitrous oxide) with and without sulfide. 

Community based profiling of the 16S rRNA gene and qPCR were used to assess the impact of sulfide on nitrifying populations. 

Metagenomic sequencing was used to evaluate how sulfide changed the functional potential in the bioreactor. 

Batch experiments using isotopically labeled nitrogen were used to measure rates of nitrification with and without sulfide.